BOB1 Antibody (BOB1/2421)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79750

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # BOB1/2421

Concentration

0.2 mg/ml

View all BOB1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 148-255) of human BOB1 protein (exact sequence is proprietary) (Uniprot: Q16633)

Localization

Nuclear

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79885)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for BOB1 Antibody (BOB1/2421)

Western Blot: BOB1 Antibody (BOB1/2421) [NBP2-79750]

Western Blot: BOB1 Antibody (BOB1/2421) [NBP2-79750]

Western Blot: BOB1 Antibody (BOB1/2421) [NBP2-79750] - Western Blot Analysis of Raji cell lysate using BOB1 Antibody (BOB1/2421).
Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2421) [NBP2-79750]

Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2421) [NBP2-79750]

Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2421) [NBP2-79750] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Antibody (BOB1/2421).
Flow Cytometry: BOB1 Antibody (BOB1/2421) [NBP2-79750]

Flow Cytometry: BOB1 Antibody (BOB1/2421) [NBP2-79750]

Flow Cytometry: BOB1 Antibody (BOB1/2421) [NBP2-79750] - Flow Cytometric Analysis of Human Raji cells using BOB1 Antibody (BOB1/2421)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
View 1 more images

Applications for BOB1 Antibody (BOB1/2421)

Application
Recommended Usage

Flow Cytometry

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: BOB1

BOB1 is a novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.

Alternate Names

B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1

Gene Symbol

POU2AF1

UniProt

Q16633 (Human)

Additional BOB1 Products

Product Documents for BOB1 Antibody (BOB1/2421)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BOB1 Antibody (BOB1/2421)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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