BOB1 Antibody (BOB1/2421) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79885
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # BOB1/2421
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 148-255) of human BOB1 protein (exact sequence is proprietary) (Uniprot: Q16633)
Localization
Nuclear
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79750).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for BOB1 Antibody (BOB1/2421) - Azide and BSA Free
Western Blot: BOB1 Antibody (BOB1/2421)Azide and BSA Free [NBP2-79885]
Western Blot: BOB1 Antibody (BOB1/2421) - Azide and BSA Free [NBP2-79885] - Western Blot Analysis of Raji cell lysate using BOB1 Antibody (BOB1/2421).Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2421) - Azide and BSA Free [NBP2-79885]
Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2421) - Azide and BSA Free [NBP2-79885] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Mouse Monoclonal Antibody (BOB1/2421).Flow Cytometry: BOB1 Antibody (BOB1/2421) - Azide and BSA Free [NBP2-79885]
Flow Cytometry: BOB1 Antibody (BOB1/2421) - Azide and BSA Free [NBP2-79885] - Flow Cytometric Analysis of Human Raji cells using BOB1 Mouse Monoclonal Antibody (BOB1/2421) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).Applications for BOB1 Antibody (BOB1/2421) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1-2 ug/ml
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: BOB1
Alternate Names
B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1
Gene Symbol
POU2AF1
Additional BOB1 Products
Product Documents for BOB1 Antibody (BOB1/2421) - Azide and BSA Free
Product Specific Notices for BOB1 Antibody (BOB1/2421) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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