Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Bora Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Bora. The immunogen is located within amino acids 410 - 460 of Bora. Amino Acid Squence: DVEREKDNNTVDMVD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bora Antibody - BSA Free

Western Blot: Bora AntibodyBSA Free [NBP1-77346]

Western Blot: Bora AntibodyBSA Free [NBP1-77346]

Western Blot: Bora Antibody [NBP1-77346] - Mouse brain tissue lysate with Bora antibody at (A) 1 and (B) 2 ug/ml.
Bora Antibody - BSA Free

Immunohistochemistry: Bora Antibody - BSA Free [NBP1-77346] -

Immunohistochemistry: Bora Antibody - BSA Free [NBP1-77346] - Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 u/mL.
Bora Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Bora Antibody - BSA Free [NBP1-77346] -

Immunocytochemistry/ Immunofluorescence: Bora Antibody - BSA Free [NBP1-77346] - Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 ug/mL.

Applications for Bora Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bora

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Long Name

Protein Aurora Borealis

Alternate Names

Aurora Borealis, C13orf34

Gene Symbol

BORA

UniProt

Q6PGQ7 (Human)

Additional Bora Products

Product Documents for Bora Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Bora Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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