BORIS Antibody (2B10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42935

Novus Biologicals, part of Bio-Techne
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NBP3-42935
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BORIS Antibodies »

Product Specifications

Immunogen

CCCTC-binding factor(zinc finger protein)-like - (Uniprot# Q8NI51)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BORIS Antibody (2B10)

BORIS Antibody (2B10)

Western Blot: BORIS Antibody (2B10) [NBP3-42935] -

Western Blot: BORIS Antibody (2B10) [NBP3-42935] - HeLa cells were subjected to SDS PAGE followed by western blot with (BORIS antibody) at dilution of 1:1000
BORIS Antibody (2B10)

Immunocytochemistry/ Immunofluorescence: BORIS Antibody (2B10) [NBP3-42935] -

Immunocytochemistry/ Immunofluorescence: BORIS Antibody (2B10) [NBP3-42935] - Immunofluorescent analysis of MCF-7 cells, using (BORIS antibody) at 1:25 dilution andRhodamine-labeled goat anti-mouse IgG (red).

Applications for BORIS Antibody (2B10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BORIS

BORIS (Brother of the Regulator of Imprinted Sites) also known as CCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm.

Alternate Names

BORISHMGB1L1, BORIS-like protein, Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein)-like, CT27MGC163358, CTCF paralog, CTCF-like protein, CTCF-T, dJ579F20.2, HMG-1L1, MGC169105, MGC169106, putative high mobility group protein 1-like 1, putative high mobility group protein B1-like 1, transcriptional repressor CTCFL, Zinc finger protein CTCF-T

Gene Symbol

CTCFL

Additional BORIS Products

Product Documents for BORIS Antibody (2B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BORIS Antibody (2B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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