BPHL Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38328

Novus Biologicals, part of Bio-Techne
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NBP2-38328
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BPHL Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RTRKPLEALYGYDYFARTCEKWVDGIRQFKHLPDGNICRHLLPRVQCPALIVHGEKDPLVPRFHADFIHKHVKGSRLHLMPEG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BPHL Antibody

Western Blot: BPHL Antibody [NBP2-38328]

Western Blot: BPHL Antibody [NBP2-38328]

Western Blot: BPHL Antibody [NBP2-38328] - Analysis using Anti-BPHL antibody NBP2-38328 (A) shows similar pattern to independent antibody NBP1-88373 (B).
Immunocytochemistry/ Immunofluorescence: BPHL Antibody [NBP2-38328]

Immunocytochemistry/ Immunofluorescence: BPHL Antibody [NBP2-38328]

Immunocytochemistry/Immunofluorescence: BPHL Antibody [NBP2-38328] - Staining of human cell line PC-3 shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BPHL Antibody [NBP2-38328]

Immunohistochemistry-Paraffin: BPHL Antibody [NBP2-38328]

Immunohistochemistry-Paraffin: BPHL Antibody [NBP2-38328] - Staining in human kidney and skeletal muscle tissues using anti-BPHL antibody. Corresponding BPHL RNA-seq data are presented for the same tissues.
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Applications for BPHL Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BPHL

The serine hydrolases, including BPHL, are defined as a functional class of hydrolytic enzymes that contain a serine residue in their active site. They can be grouped into subfamilies that contain closely related members in terms of substrate specificity or amino acid sequence similarity (Puente and Lopez-Otin, 1995 [PubMed 7759552]).[supplied by OMIM]

Alternate Names

biphenyl hydrolase-like (serine hydrolase), Biphenyl hydrolase-like protein, Biphenyl hydrolase-related protein, Bph-rp, Breast epithelial mucin-associated antigen, EC 3.1, MCNAA, MGC125930, MGC41865, VACVase, valacyclovir hydrolase, Valacyclovirase

Gene Symbol

BPHL

UniProt

Q86WA6 (Human)

Additional BPHL Products

Product Documents for BPHL Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BPHL Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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