Brachyury Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87598

Novus Biologicals, part of Bio-Techne
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NBP1-87598
NBP1-87598-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Brachyury Antibodies »

Product Summary for Brachyury Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QQPGYSQWGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (87%)

Marker

Mesoderm Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brachyury Antibody

Immunocytochemistry/ Immunofluorescence: Brachyury Antibody [NBP1-87598]

Immunocytochemistry/ Immunofluorescence: Brachyury Antibody [NBP1-87598]

Immunocytochemistry/Immunofluorescence: Brachyury Antibody [NBP1-87598] - Staining of human cell line HeLa shows localization to nucleus. Antibody staining is shown in green.

Applications for Brachyury Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brachyury

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Gene Symbol

TBXT

Additional Brachyury Products

Product Documents for Brachyury Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Brachyury Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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