Brachyury Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24676

Novus Biologicals, part of Bio-Techne
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NBP2-24676
NBP2-24676SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse

Predicted:

Canine (93%), Monkey (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Brachyury Antibodies »

Product Specifications

Immunogen

A portion of amino acids 350-400 of human Brachyury was used as the immunogen.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28132888).

Marker

Mesoderm Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brachyury Antibody

Western Blot: Brachyury Antibody [NBP2-24676]

Western Blot: Brachyury Antibody [NBP2-24676]

Western Blot: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in U87 cell lysate. Lane 1 shows pre-immune sera, lane 2 shows this antibody tested at 2 ug/mL.
Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676]

Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676]

Immunohistochemistry-Paraffin: Brachyury Antibody [NBP2-24676] - Analysis of Brachyury in FFPE human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/mL.

Applications for Brachyury Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:1000

Immunohistochemistry-Paraffin

5 ug/mL

Western Blot

1 - 3 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brachyury

Brachyury / Bry is encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Gene Symbol

TBXT

UniProt

O15178

Additional Brachyury Products

Product Documents for Brachyury Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Brachyury Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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