BRCA1 Antibody (8F7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41186

Novus Biologicals, part of Bio-Techne
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NBP1-41186
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BRCA1 Antibodies »

Product Specifications

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 341-748.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear/Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

208 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BRCA1 Antibody (8F7)

Western Blot: BRCA1 Antibody (8F7) [NBP1-41186]

Western Blot: BRCA1 Antibody (8F7) [NBP1-41186]

Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunohistochemistry: BRCA1 Antibody (8F7) [NBP1-41186]

Immunohistochemistry: BRCA1 Antibody (8F7) [NBP1-41186]

Immunohistochemistry: BRCA1 Antibody (8F7) [NBP1-41186] - Paraffin-embedded Breast ca, using BRCA1 antibody at 1:200 dilution.
BRCA1 Antibody (8F7)

Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] -

Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382 was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for BRCA1 Antibody (8F7)

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000
Application Notes
Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRCA1

BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Entrez Gene IDs

672 (Human)

Gene Symbol

BRCA1

UniProt

P38398 (Human)

Additional BRCA1 Products

Product Documents for BRCA1 Antibody (8F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRCA1 Antibody (8F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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