Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BRCA1 Antibodies »

Product Specifications

Immunogen

Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Breast Cancer Gene 1 (GeneID 672) around serine 1466 according to the numbering given in entry NP_009225.1.

Reactivity Notes

100% Sequence Identity: Gorilla, Chimpanzee

Modification

p Ser1466

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRCA1 [p Ser1466] Antibody

Western Blot: BRCA1 [p Ser1466] Antibody [NB100-228]

Western Blot: BRCA1 [p Ser1466] Antibody [NB100-228]

Western Blot: BRCA1 [p Ser1466] Antibody [NB100-228] - Whole Cell Lysate (25ug/lane) from Hela cells treated with 10 Gy IR or untreated. Antibody diluted 1:2000

Applications for BRCA1 [p Ser1466] Antibody

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
The investigator should determine the optimal dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRCA1

BRCA 1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA 1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA 1 function confers an increased susceptibility for both breast and ovarian cancer.

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Entrez Gene IDs

672 (Human)

Gene Symbol

BRCA1

UniProt

NP_009225.1. (Human)

Additional BRCA1 Products

Product Documents for BRCA1 [p Ser1466] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRCA1 [p Ser1466] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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