BRCA2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88361
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VHPISLSSSKCHDSVVSMFKIENHNDKTVSEKNNKCQLILQNNIEMTTGTFVEEITENYKRNTENEDNKYTAASRNSHNLEFDGSDSSKNDTVCIHKDETDLLFTDQHNICLKLSGQFMKEGNTQIKEDLS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRCA2 Antibody
![Immunocytochemistry/ Immunofluorescence: BRCA2 Antibody [NBP1-88361]](https://resources.bio-techne.com/images/products/BRCA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88361-img0011.jpg "Immunocytochemistry/ Immunofluorescence: BRCA2 Antibody [NBP1-88361]")
Immunocytochemistry/ Immunofluorescence: BRCA2 Antibody [NBP1-88361]
Immunocytochemistry/Immunofluorescence: BRCA2 Antibody [NBP1-88361] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.![Flow Cytometry: BRCA2 Antibody [NBP1-88361]](https://resources.bio-techne.com/images/products/BRCA2-Antibody-Flow-Cytometry-NBP1-88361-img0012.jpg "Flow Cytometry: BRCA2 Antibody [NBP1-88361]")
Flow Cytometry: BRCA2 Antibody [NBP1-88361]
Flow Cytometry: BRCA2 Antibody [NBP1-88361] - DNA damage induced by low-dose radiation and cfDNAox and cfDNAoxR fragments activates reparation of nuclear DNA. (a) (1) Flow cytometry detection of BRCA2 in irradiated (10cGy) and control cells stained with anti-BRCA2 antibodies and secondary FITC-conjugated antibodies. Gate R encircles the fraction of MSCs with elevated values of FL1- BRCA2; (2) distribution of cells with varying BRCA2 contents. Image collected and cropped by CiteAb from the following publication (hindawi.com/journals/omcl/2017/9515809/), licensed under a CC-BY license.Applications for BRCA2 Antibody
Application
Recommended Usage
Flow Cytometry
Reported in scientific literature (PMID: 28904740)
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BRCA2
Long Name
Breast Cancer 2
Alternate Names
BRCA1/BRCA2-containing complex, subunit 2, BRCC2, breast and ovarian cancer susceptibility gene, early onset, breast cancer 2 tumor suppressor, breast cancer 2, early onset, breast cancer susceptibility protein BRCA2, breast cancer type 2 susceptibility protein, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1Fanconi anemia, complementation group D1, Fanconi anemia group D1 protein, GLM3, PNCA2
Entrez Gene IDs
675 (Human)
Gene Symbol
BRCA2
UniProt
Additional BRCA2 Products
Product Documents for BRCA2 Antibody
Product Specific Notices for BRCA2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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