BRD4 Antibody (1E5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26475
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1E5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for BRD4 Antibody (1E5)
Immunogen
A synthesized peptide derived from Human BRD4 [UniProt O60885]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRD4 Antibody (1E5)
Immunohistochemistry: BRD4 Antibody (1E5) [NBP3-26475] -
Immunohistochemistry: BRD4 Antibody (1E5) [NBP3-26475] - Image of BRD4 Antibody (1E5) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: BRD4 Antibody (1E5) [NBP3-26475] -
Immunohistochemistry: BRD4 Antibody (1E5) [NBP3-26475] - Image of BRD4 Antibody (1E5) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: BRD4 Antibody (1E5) [NBP3-26475] -
Western Blot: BRD4 Antibody (1E5) [NBP3-26475] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate.All lanes: BRD4 Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 153, 81, 89 kDa
Observed band size: 153 kDa
Applications for BRD4 Antibody (1E5)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: BRD4
Long Name
Bromodomain Containing 4
Alternate Names
HUNK1, HUNKI, MCAP
Gene Symbol
BRD4
Additional BRD4 Products
Product Documents for BRD4 Antibody (1E5)
Product Specific Notices for BRD4 Antibody (1E5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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