BRD4 Antibody (CL1118) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44014

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1118

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all BRD4 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O60885, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for BRD4 Antibody (CL1118) - Azide and BSA Free

BRD4 Antibody (CL1118) - Azide and BSA Free Western Blot: BRD4 Antibody [NBP3-44014]

Western Blot: BRD4 Antibody [NBP3-44014]

Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
BRD4 Antibody (CL1118) - Azide and BSA Free Immunohistochemistry-Paraffin: BRD4 Antibody [NBP3-44014]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP3-44014]

Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
BRD4 Antibody (CL1118) - Azide and BSA Free Immunohistochemistry-Paraffin: BRD4 Antibody [NBP3-44014]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP3-44014]

Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and in glomeruli.
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Applications for BRD4 Antibody (CL1118) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody (CL1118) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRD4 Antibody (CL1118) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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