BRD4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18874
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRD4 Antibody
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874]
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DABImmunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874]
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:1,000 (1 ug/ml). Detection: DABImmunoprecipitation: BRD4 Antibody [NBP1-18874]
Immunoprecipitation: BRD4 Antibody [NBP1-18874] - Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD4 antibody NBP1-18874 used for IP at 3 ug per reaction. BRD4 was also immunoprecipitated by a different rabbit anti-BRD4 antibody and rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (NBP2-76393). For blotting immunoprecipitated BRD4, NBP2-76393 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 1 seconds.Applications for BRD4 Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000-1:5000
Immunohistochemistry-Paraffin
1:1000-1:5000
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BRD4
Long Name
Bromodomain Containing 4
Alternate Names
HUNK1, HUNKI, MCAP
Entrez Gene IDs
23476 (Human)
Gene Symbol
BRD4
UniProt
Additional BRD4 Products
Product Documents for BRD4 Antibody
Product Specific Notices for BRD4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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