BRD4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38931

Novus Biologicals, part of Bio-Techne
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NBP2-38931
NBP2-38931-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BRD4 Antibodies »

Product Summary for BRD4 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PPFIATQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD4 Antibody

Immunocytochemistry/ Immunofluorescence: BRD4 Antibody [NBP2-38931]

Immunocytochemistry/ Immunofluorescence: BRD4 Antibody [NBP2-38931]

Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NBP2-38931] - Staining of human cell line PC-3 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
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Applications for BRD4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 -1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

UniProt

O60885

Additional BRD4 Products

Product Documents for BRD4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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