BRD4 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15953
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Rat
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600-722 of human BRD4 (NP_055114.1). ESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETGPA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRD4 Antibody - Azide and BSA Free
![Western Blot: BRD4 AntibodyAzide and BSA Free [NBP3-15953]](https://resources.bio-techne.com/images/products/BRD4-Antibody-Western-Blot-NBP3-15953-img0003.jpg "Western Blot: BRD4 AntibodyAzide and BSA Free [NBP3-15953]")
Western Blot: BRD4 AntibodyAzide and BSA Free [NBP3-15953]
Western Blot: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts of HeLa cells, using BRD4 antibody (NBP3-15953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry-Paraffin: BRD4 Antibody - Azide and BSA Free [NBP3-15953]](https://resources.bio-techne.com/images/products/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP3-15953-img0002.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody - Azide and BSA Free [NBP3-15953]")
Immunohistochemistry-Paraffin: BRD4 Antibody - Azide and BSA Free [NBP3-15953]
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP3-15953] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] BRD4 Rabbit pAb (NBP3-15953) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Knockout Validated: BRD4 Antibody - Azide and BSA Free [NBP3-15953]](https://resources.bio-techne.com/images/products/BRD4-Antibody-Knockout-Validated-NBP3-15953-img0001.jpg "Knockout Validated: BRD4 Antibody - Azide and BSA Free [NBP3-15953]")
Knockout Validated: BRD4 Antibody - Azide and BSA Free [NBP3-15953]
Knockout Validated: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts from normal (control) and BRD4 Rabbit pAb knockout (KO) HeLa cells, using BRD4 Rabbit pAb antibody (NBP3-15953) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Applications for BRD4 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BRD4
Long Name
Bromodomain Containing 4
Alternate Names
HUNK1, HUNKI, MCAP
Gene Symbol
BRD4
Additional BRD4 Products
Product Documents for BRD4 Antibody - Azide and BSA Free
Product Specific Notices for BRD4 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov