BRD4 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15953

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BRD4 Antibodies »

Product Summary for BRD4 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-722 of human BRD4 (NP_055114.1). ESEEEDKCKPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETGPA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD4 Antibody - Azide and BSA Free

Western Blot: BRD4 AntibodyAzide and BSA Free [NBP3-15953]

Western Blot: BRD4 AntibodyAzide and BSA Free [NBP3-15953]

Western Blot: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts of HeLa cells, using BRD4 antibody (NBP3-15953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: BRD4 Antibody - Azide and BSA Free [NBP3-15953]

Immunohistochemistry-Paraffin: BRD4 Antibody - Azide and BSA Free [NBP3-15953]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP3-15953] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] BRD4 Rabbit pAb (NBP3-15953) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Knockout Validated: BRD4 Antibody - Azide and BSA Free [NBP3-15953]

Knockout Validated: BRD4 Antibody - Azide and BSA Free [NBP3-15953]

Knockout Validated: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts from normal (control) and BRD4 Rabbit pAb knockout (KO) HeLa cells, using BRD4 Rabbit pAb antibody (NBP3-15953) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for BRD4 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BRD4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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