BRIP1/FANCJ Antibody (pp15-IB4)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-189

Novus Biologicals, part of Bio-Techne
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NB100-189
NB100-189SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # pp15-IB4

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all BRIP1/FANCJ Antibodies »

Product Specifications

Immunogen

A peptide corresponding to the C-terminus of human FANCJ (within amino acids 1200-1249). [SwissProt# Q9BX63]

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BRIP1/FANCJ Antibody (pp15-IB4)

Western Blot: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189]

Western Blot: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189]

Western Blot: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Used to detect BRIP1/FANCJ in 293 cell lysate.
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189]

Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189]

Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody (pp15-IB4) [NB100-189] - Analysis of a FFPE tissue section of the human breast cancer using 1:200 dilution of BRIP1/FANCJ antibody (NB100-189). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated a nuclear stain in ductal cells with some cytoplasmic localization.

Applications for BRIP1/FANCJ Antibody (pp15-IB4)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRIP1/FANCJ

FANCJ (Fanconi anemia group J protein) was originally identified as a protein which binds BRCT domains of BRCA1 and was named BACH1 (BRCA1-Associated C-terminal Helicase1) or BRIP1 (BRCA1 Interacting Protein C-terminal Helicase1), and after its identification as a component of Fanconi anemia pathway, it was renamed as FANCJ. It is expressed ubiquitously with highest levels in testes and sub-cellularly, it localizes to the nucleus. FANCJ is a DNA-dependent ATPase with 5' to 3' DNA helicase activity which is essential for genetic and structural integrity of genome. FANCJ acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination, and is implicated in repairing DNA double-strand breaks through homologous recombination in FANCJ-BRCA1 interactions dependent manner. Cell-cycle regulated phosphorylation of FANCJ is essential for BRCA1 interaction and 4Fe-4S iron-sulfur-binding is required for its helicase activity. The interaction of FANCJ with proteins including MutLalpha complex and BRCA1, suggests its importance in other pathways of DNA repair and in diseases such as colon and breast cancer. Defective FANCJ causes chromosome instability disorder Fanconi anemia complementation group J.

Long Name

BRCA1 Interacting Protein C-terminal Helicase 1

Alternate Names

BACH1, FACJ, FANCJ

Entrez Gene IDs

83990 (Human)

Gene Symbol

BRIP1

UniProt

Q9BX63

Additional BRIP1/FANCJ Products

Product Documents for BRIP1/FANCJ Antibody (pp15-IB4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BRIP1/FANCJ Antibody (pp15-IB4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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