Bromodeoxyuridine/BrdU Antibody (BU20a) [Janelia Fluor® 635]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34567JF635

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Janelia Fluor 635

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # BU20a

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Bromodeoxyuridine/BrdU Antibodies »

Product Summary for Bromodeoxyuridine/BrdU Antibody (BU20a) [Janelia Fluor® 635]

Immunogen

Bromodeoxyuridine/BrdU conjugated to KLH

Localization

Nuclear

Specificity

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Marker

Proliferation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Bromodeoxyuridine/BrdU Antibody (BU20a) [Janelia Fluor® 635]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Bromodeoxyuridine/BrdU

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU Antibody (BU20a) [Janelia Fluor® 635]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BU20a) [Janelia Fluor® 635]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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