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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 35 and 70 of basic transcription factor 3 using the numbering given in entry NP_001032726.1 (GeneID 689).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BTF3 Antibody - BSA Free

Immunohistochemistry: BTF3 Antibody [NBP1-28700]

Immunohistochemistry: BTF3 Antibody [NBP1-28700]

Immunohistochemistry: BTF3 Antibody [NBP1-28700] - Sample: FFPE section of human breast carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- BTF3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: BTF3 Antibody [NBP1-28700]

Immunoprecipitation: BTF3 Antibody [NBP1-28700]

Immunoprecipitation: BTF3 Antibody [NBP1-28700] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTF3 antibody NBP1-28700 used for IP at 10 ug/mg lysate. BTF3 was also immunoprecipitated by rabbit anti-BTF3 antibody NBP1-28716, which recognizes an upstream epitope. For blotting immunoprecipitated BTF3, NBP1-28716 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for BTF3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate
Application Notes
It is not recommended for western analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BTF3

Basic transcription factor 3 (BTF3) is a general transcription factor that forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation.

Long Name

Basic Transcription Factor 3

Alternate Names

BETA-NAC, NACB

Entrez Gene IDs

689 (Human)

Gene Symbol

BTF3

Additional BTF3 Products

Product Documents for BTF3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BTF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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