BTK Antibody (7F12H4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80605

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7F12H4

Format

Azide and BSA Free

Concentration

2.2 mg/ml

View all BTK Antibodies »

Product Specifications

Immunogen

Purified recombinant C terminal fragment of BTK expressed in E. coli. [UniProt# Q06187]

Localization

Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note: In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BTK Antibody (7F12H4) - Azide and BSA Free

Western Blot: BTK Antibody (7F12H4)Azide and BSA Free [NBP2-80605]

Western Blot: BTK Antibody (7F12H4)Azide and BSA Free [NBP2-80605]

Western Blot: BTK Antibody (7F12H4) - Azide and BSA Free [NBP2-80605] - Analysis of BTK expression in 2) Raji, 3) A431 whole cell lysates and 4) human tonsil tissue extract. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: BTK Antibody (7F12H4) - Azide and BSA Free [NBP2-80605]

Immunocytochemistry/ Immunofluorescence: BTK Antibody (7F12H4) - Azide and BSA Free [NBP2-80605]

Immunocytochemistry/Immunofluorescence: BTK Antibody (7F12H4) - Azide and BSA Free [NBP2-80605] - The BTK antibody was tested in A431 cells against DyLight 488 (Green). Actin and nuclei were counterstained for Phalloidin (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: BTK Antibody (7F12H4) - Azide and BSA Free [NBP2-80605]

Immunohistochemistry-Paraffin: BTK Antibody (7F12H4) - Azide and BSA Free [NBP2-80605]

Immunohistochemistry-Paraffin: BTK Antibody (7F12H4) - Azide and BSA Free [NBP2-80605] - Immunohistochemical analysis of paraffin-embedded human lymph-node tissues (left) and human lymph follicle tissues (right), showing cytoplasmic and membrane localization using BTK mouse mAb with DAB staining. Image from the standard format of this antibod
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Applications for BTK Antibody (7F12H4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This BTK (7F12H4) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin-embedded sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

2.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BTK

BTK, also known as Brutons tyrosine kinase, is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Long Name

Bruton Agammaglobulinemia Tyrosine Kinase

Alternate Names

AGMX1, IMD1, PSCTK1, XLA

Gene Symbol

BTK

Additional BTK Products

Product Documents for BTK Antibody (7F12H4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BTK Antibody (7F12H4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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