BTK Antibody (OTI3A2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02472

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A2

Concentration

0.5 mg/ml

View all BTK Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

76.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BTK Antibody (OTI3A2)

Western Blot: BTK Antibody (OTI3A2) [NBP2-02472]

Western Blot: BTK Antibody (OTI3A2) [NBP2-02472]

Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
Immunohistochemistry-Paraffin: BTK Antibody (OTI3A2) [NBP2-02472]

Immunohistochemistry-Paraffin: BTK Antibody (OTI3A2) [NBP2-02472]

Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded prostate tissue using anti-BTK mouse monoclonal antibody.
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Applications for BTK Antibody (OTI3A2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BTK

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement.

Long Name

Bruton Agammaglobulinemia Tyrosine Kinase

Alternate Names

AGMX1, IMD1, PSCTK1, XLA

Entrez Gene IDs

695 (Human)

Gene Symbol

BTK

Additional BTK Products

Product Documents for BTK Antibody (OTI3A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BTK Antibody (OTI3A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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