BTK [p Tyr551] Antibody (SR2188)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21550

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21550-100ul
NBP3-21550-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2188

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BTK Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-BTK [Tyr551] (Uniprot #: Q06187)

Modification

p Tyr 551

Specificity

Detects endogenous levels of phospho-BTK [Tyr551]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BTK [p Tyr551] Antibody (SR2188)

Western Blot: BTK [p Tyr 551] Antibody (SR2188) [NBP3-21550] -

Western Blot: BTK [p Tyr 551] Antibody (SR2188) [NBP3-21550] - Western blot analysis using NBP3-21550 in Ramos cell lysate treated with Pervanadate.

Applications for BTK [p Tyr551] Antibody (SR2188)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BTK

Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. All members of the BTK/Tec family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Long Name

Bruton Agammaglobulinemia Tyrosine Kinase

Alternate Names

AGMX1, IMD1, PSCTK1, XLA

Gene Symbol

BTK

Additional BTK Products

Product Documents for BTK [p Tyr551] Antibody (SR2188)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BTK [p Tyr551] Antibody (SR2188)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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