Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all BTK Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to 16 amino acids near the amino-terminus of human BTK. The immunogen is located within amino acids 20 - 70 of BTK. Amino Acid Squence: SYYEYDFERGRRGSKK

Specificity

Multiple isoforms of BTK are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BTK Antibody - BSA Free

Western Blot: BTK AntibodyBSA Free [NBP1-76596]

Western Blot: BTK AntibodyBSA Free [NBP1-76596]

Western Blot: BTK Antibody [NBP1-76596] - Analysis of BTK in U937 cell lysate with anti-BTK using NBP1-76596 at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: BTK Antibody - BSA Free [NBP1-76596]

Immunocytochemistry/ Immunofluorescence: BTK Antibody - BSA Free [NBP1-76596]

Immunocytochemistry/Immunofluorescence: BTK Antibody [NBP1-76596] - Daudi cells with BTK antibody at 10 ug/ml.
BTK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BTK Antibody - BSA Free [NBP1-76596] -

Immunocytochemistry/ Immunofluorescence: BTK Antibody - BSA Free [NBP1-76596] - Immunofluorescence of BTK in Daudi cells with BTK antibody at 10 ug/mL.
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Applications for BTK Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BTK

Bruton's tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked agamma-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage. It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9. The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens. BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.

Long Name

Bruton Agammaglobulinemia Tyrosine Kinase

Alternate Names

AGMX1, IMD1, PSCTK1, XLA

Gene Symbol

BTK

UniProt

Q06187

Additional BTK Products

Product Documents for BTK Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BTK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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