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BTLA/CD272 Antibody (HMBT-6B2) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81117CL1

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-81117CL1

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Block/Neutralize, Flow Cytometry, Functional, Immunoblotting, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Recombinant Monoclonal Armenian Hamster IgG Kappa Clone # HMBT-6B2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for BTLA/CD272 Antibody (HMBT-6B2) [CoraFluor™ 1]

Immunogen

This antibody was raised by immunising Armenian hamsters with mouse BTLA/CD272:Fc fusion protein.

Specificity

This antibody is specific for B and T lymphocyte attenuator (BTLA), which functions as a negative regulator of T cell activation and proliferation, and attenuates B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG Kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for BTLA/CD272 Antibody (HMBT-6B2) [CoraFluor™ 1]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: BTLA

BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM]

Long Name

B- And T-Lymphocyte Attenuator

Alternate Names

CD272

Gene Symbol

BTLA

Additional BTLA Products

Product Documents for BTLA/CD272 Antibody (HMBT-6B2) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BTLA/CD272 Antibody (HMBT-6B2) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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