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Bub1 Antibody (9C11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15170

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15170

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9C11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Bub1 Antibody (9C11)

Immunogen

A synthesized peptide derived from human Bub1. UniProt ID (O43683).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bub1 Antibody (9C11)

Western Blot: Bub1 Antibody (9C11) [NBP3-15170]

Western Blot: Bub1 Antibody (9C11) [NBP3-15170]

Western Blot: Bub1 Antibody (9C11) [NBP3-15170] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: Bub1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 123, 116, 120 KDa. Observed band size: 130 KDa.

Applications for Bub1 Antibody (9C11)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Bub1

BUB1 encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer.

Long Name

BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase

Gene Symbol

BUB1

Additional Bub1 Products

Product Documents for Bub1 Antibody (9C11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Bub1 Antibody (9C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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