c-Abl Antibody (M209)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23312
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M209
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone (M209) was generated from a recombinant protein corresponding to the C-terminal region of human c-Abl. [UniProt# P00519]
Specificity
This antibody detects a 145 kDa protein corresponding to c-Abl on SDS-PAGE immunoblots of human K-562 and HL-60 cells, and mouse ANN-1 cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Scientific Data Images for c-Abl Antibody (M209)
Western Blot: c-Abl Antibody (M209) [NBP3-23312] -
Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).
Western Blot: c-Abl Antibody (M209) [NBP3-23312] -
Western Blot: c-Abl Antibody (M209) [NBP3-23312] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).Applications for c-Abl Antibody (M209)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS and 0.2% gelatin
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: c-Abl
Long Name
Abelson Murine Leukemia Viral Oncogene Homolog 1
Alternate Names
ABL1, BCR-ABL1, bcr/abl, cAbl, JTK7
Gene Symbol
ABL1
Additional c-Abl Products
Product Specific Notices for c-Abl Antibody (M209)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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