c-Abl [p Tyr245] Antibody

Biological Validation

Validated:

Unconjugated

Polyclonal Rabbit IgG

Please see the vial label for concentration. If unlisted please contact technical services.

Phospho-c-Abl (Tyr-245) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 245 of human c-Abl. This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein. [UniProt# P00519]

p Tyr245

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Abl (Tyr-245) peptide (without carrier). On SDS-PAGE immunoblots of K-562 treated with pervanadate, the antibody detects a 145 kDa protein corresponding to c-Abl and a 210 kDa band corresponding to BCR-Abl. In addition, this antibody detects a 145 kDa band in Jurkat cells treated with pervanadate.

Polyclonal

Rabbit

IgG

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Stable for at least 1 year at -20°C.

Antigen Affinity-purified

PBS, 1 mg/ml BSA and 50% glycerol

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C.