c-Abl [p Tyr412] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23318
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Phospho-c-Abl (Tyr-412) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 412 of human c-Abl. This peptide sequence has high homology to the conserved site in rat and mouse c-Abl, as well as in viral Abl and BCR-Abl fusion protein. [UniProt# P00519]
Modification
p Tyr412
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-c-Abl (Tyr-245) peptide before affinity purification using phospho-c-Abl (Tyr-412) peptide. On SDS-PAGE immunoblots of K-562 treated with pervanadate the antibody detects a 145 kDa protein corresponding to c-Abl and a 210 kDa band corresponding to BCR-Abl.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for c-Abl [p Tyr412] Antibody
![c-Abl [p Tyr412] Antibody](https://resources.bio-techne.com/images/products/nbp3-23318_rabbit-c-abl-p-tyr412-pab-19920231915414.jpg "Western Blot: c-Abl [p Tyr412] Antibody [NBP3-23318] -")
Western Blot: c-Abl [p Tyr412] Antibody [NBP3-23318] -
Western Blot: c-Abl [p Tyr412] Antibody [NBP3-23318] - Western blot analysis of K-562 cells treated with pervanadate (1 mM) for 30 minutes (lanes 1, 3, & 5). Some lanes were treated with alkaline phosphatase to remove phosphorylation on c-Abl (lanes 2, 4, & 6), then the blots were probed with anti-c-Abl (lanes 1 & 2), anti-c-Abl (Tyr-412) (NBP3-23318; lanes 3 & 4), or anti-c-Abl (Tyr-245) (NBP3-23313; lanes 5 & 6).Applications for c-Abl [p Tyr412] Antibody
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: c-Abl
Long Name
Abelson Murine Leukemia Viral Oncogene Homolog 1
Alternate Names
ABL1, BCR-ABL1, bcr/abl, cAbl, JTK7
Gene Symbol
ABL1
Additional c-Abl Products
Product Documents for c-Abl [p Tyr412] Antibody
Product Specific Notices for c-Abl [p Tyr412] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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