c-jun Antibody (10D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26186

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26186-100ul
NBP3-26186-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10D4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all c-Jun Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human c-jun [UniProt P05412]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for c-jun Antibody (10D4)

c-jun Antibody (10D4)

Immunohistochemistry: c-jun Antibody (10D4) [NBP3-26186] -

Immunohistochemistry: c-jun Antibody (10D4) [NBP3-26186] - Image of c-jun Antibody (10D4) diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
c-jun Antibody (10D4)

Western Blot: c-jun Antibody (10D4) [NBP3-26186] -

Western Blot: c-jun Antibody (10D4) [NBP3-26186] - Positive Western Blot detected in: 293 whole cell lysate.
All lanes: c-jun Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 36 kDa
Observed band size: 43 kDa

Applications for c-jun Antibody (10D4)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: c-Jun

The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Gene Symbol

JUN

Additional c-Jun Products

Product Documents for c-jun Antibody (10D4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for c-jun Antibody (10D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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