c-jun [p Ser73] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13304

Novus Biologicals, part of Bio-Techne
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NBP3-13304
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Porcine (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all c-Jun Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser73 of human c-jun. The exact sequence is proprietary.

Modification

p Ser 73

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for c-jun [p Ser73] Antibody

Western Blot: c-jun [p Ser73] Antibody [NBP3-13304]

Western Blot: c-jun [p Ser73] Antibody [NBP3-13304]

Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: c-jun [p Ser73] Antibody [NBP3-13304]

Immunocytochemistry/ Immunofluorescence: c-jun [p Ser73] Antibody [NBP3-13304]

Immunocytochemistry/Immunofluorescence: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.
Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304]

Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304]

Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for c-jun [p Ser73] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Jun

The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Gene Symbol

JUN

Additional c-Jun Products

Product Documents for c-jun [p Ser73] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for c-jun [p Ser73] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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