c-Myc [p Thr58, p Ser62] Antibody (4E9) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26403
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4E9
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human c-Myc [p Thr58, p Ser62] [UniProt P01106]
Modification
p Thr 58, p Ser 62
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for c-Myc [p Thr58, p Ser62] Antibody (4E9) - BSA Free
![c-Myc [p Thr58, p Ser62] Antibody (4E9)](https://resources.bio-techne.com/images/products/nbp3-26403_rabbit-c-myc-p-thr-58-p-ser-62-mab-4e9-28220241152519.jpg "Western Blot: c-Myc [p Thr58, p Ser62] Antibody (4E9) [NBP3-26403] -")
Western Blot: c-Myc [p Thr58, p Ser62] Antibody (4E9) [NBP3-26403] -
Western Blot: c-Myc [p Thr58, p Ser62] Antibody (4E9) [NBP3-26403] - Positive Western Blot detected in HepG2 whole cell lysate (treated with EGF or not).All lanes: c-Myc [p Thr58, p Ser62] Antibody at 0.99ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 57 KDa
Observed band size: 57 KDa
Applications for c-Myc [p Thr58, p Ser62] Antibody (4E9) - BSA Free
Application
Recommended Usage
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: c-Myc
A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).
References
1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07
2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934
3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892
Long Name
v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
Alternate Names
cMyc, Myc, Myc2, Niard, Nird
Gene Symbol
MYC
Additional c-Myc Products
Product Documents for c-Myc [p Thr58, p Ser62] Antibody (4E9) - BSA Free
Product Specific Notices for c-Myc [p Thr58, p Ser62] Antibody (4E9) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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