c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52636
Recombinant Monoclonal Antibody.
![Western Blot: c-Myc Antibody (9E10)ChimericAzide and BSA Free [NBP2-52636]](https://resources.bio-techne.com/images/products/c-Myc-Antibody-9E10-Western-Blot-NBP2-52636-img0001.jpg "Western Blot: c-Myc Antibody (9E10)ChimericAzide and BSA Free [NBP2-52636]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Drosophila, Yeast
Cited:
Human, Mouse, Rat, Insect - Drosophila, Yeast
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Chromatin Immunoprecipitation (ChIP), IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 9E10
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to aa 408-439 from C-terminus of human c-Myc.
Reactivity Notes
Use in Drosophila reported in scientific literature (PMID:31767639).
Specificity
Epitope sequence, EQKLISEEDL, located in aa 410-419 of human c-myc protein.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free
Western Blot: c-Myc Antibody (9E10)ChimericAzide and BSA Free [NBP2-52636]
Western Blot: c-Myc Antibody (9E10) [NBP2-52636] - Analysis of HEK293 lysate spiked (A) and unspiked (B) with 5 ng multitag protein (35 ug protein in RIPA buffer). Lane C contains 25 ng purified multitag protein as positive control. Right Panel: (1 ug/ml) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. For both, primary incubation was 1 hour. Detected by chemiluminescence.![ELISA: c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free [NBP2-52636]](https://resources.bio-techne.com/images/products/c-Myc-Antibody-9E10-NBP2-52636-img0002.jpg "ELISA: c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free [NBP2-52636]")
ELISA: c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free [NBP2-52636]
ELISA: c-Myc Antibody (9E10) [NBP2-52636] - Supporting data for Anti-c-myc epitope tag [9E10]. ELISA of anti-epitope tag antibodies on multi-tag protein. Plate coated with 5ug/ml of Multitag protein. Primary antibody loaded at 1000ng/ml in first well and an 11 point serial dilution performed in duplicate for each antibody with last well left blank. Signal detection was performed using HRP-conjugated goat anti-mouse.Applications for c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Frozen
1:10 - 1:500
Immunohistochemistry-Paraffin
1:10 - 1:500
Immunoprecipitation
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This is a chimeric rabbit antibody, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: c-Myc
A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).
References
1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07
2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934
3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892
Long Name
v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
Alternate Names
cMyc, Myc, Myc2, Niard, Nird
Gene Symbol
MYC
Additional c-Myc Products
Product Documents for c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free
Product Specific Notices for c-Myc Antibody (9E10) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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