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c-Rel Antibody (1E7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37593

Novus Biologicals, part of Bio-Techne
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NBP2-37593
NBP2-37593-0.025ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1E7

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

Purified recombinant fragment of human c-Rel expressed in E. Coli.;

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for c-Rel Antibody (1E7) - BSA Free

Western Blot: c-Rel Antibody (1E7)BSA Free [NBP2-37593]

Western Blot: c-Rel Antibody (1E7)BSA Free [NBP2-37593]

Western Blot: c-Rel Antibody (1E7) [NBP2-37593] - Analysis using c-Rel mouse mAb against Jurkat (1), NIH/3T3 (2), Hela (3), HEK293 (4) and RAJI (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: c-Rel Antibody (1E7) - BSA Free [NBP2-37593]

Immunocytochemistry/ Immunofluorescence: c-Rel Antibody (1E7) - BSA Free [NBP2-37593]

Immunocytochemistry/Immunofluorescence: c-Rel Antibody (1E7) [NBP2-37593] - Analysis of U251 cells using c-Rel mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: c-Rel Antibody (1E7) - BSA Free [NBP2-37593]

Immunohistochemistry-Paraffin: c-Rel Antibody (1E7) - BSA Free [NBP2-37593]

Immunohistochemistry-Paraffin: c-Rel Antibody (1E7) [NBP2-37593] - Analysis of endometrial cancer tissues (left) and liver cancer tissues (right) using c-Rel mouse mAb with DAB staining.

Applications for c-Rel Antibody (1E7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Rel

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA (MIM 164014), RELB (604758), NFKB1 (MIM 164011), and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain,' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein, 2007 [PubMed 18037997]).[supplied by OMIM]

Long Name

Cellular Repressor of E1A-stimulated Genes

Alternate Names

cRel, REL

Gene Symbol

REL

Additional c-Rel Products

Product Documents for c-Rel Antibody (1E7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for c-Rel Antibody (1E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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