C9orf72 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47146

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all C9orf72 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal sequence of the human C9orf72 protein [UniProt Q96LT7].

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for C9orf72 Antibody - BSA Free

Western Blot: C9orf72 AntibodyBSA Free [NBP2-47146]

Western Blot: C9orf72 AntibodyBSA Free [NBP2-47146]

Western Blot: C9orf72 Antibody [NBP2-47146] - Western Blot Image of anti-C9orf72. Whole cell protein from SK-N-SH (lane 1) and SH-SY-5Y (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-hnRNP in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: C9orf72 Antibody - BSA Free [NBP2-47146]

Immunocytochemistry/ Immunofluorescence: C9orf72 Antibody - BSA Free [NBP2-47146]

Immunocytochemistry/Immunofluorescence: C9orf72 Antibody [NBP2-47146] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-C9orf72 (NBP2-47146) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: C9orf72 Antibody - BSA Free [NBP2-47146]

Immunohistochemistry-Paraffin: C9orf72 Antibody - BSA Free [NBP2-47146]

Immunohistochemistry-Paraffin: C9orf72 Antibody [NBP2-47146] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using C9orf72 antibody (NBP2-47146) at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated strong/specific cytoplasmic signal in the neurons and other cell types in the tested section.

Applications for C9orf72 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:300

Western Blot

2.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: C9orf72

Long Name

Chromosome 9 Open Reading Frame 72

Alternate Names

DENNL72, FTDALS1

Gene Symbol

C9orf72

UniProt

Q96LT7 (Human)

Additional C9orf72 Products

Product Documents for C9orf72 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for C9orf72 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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