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CA125/MUC16 Antibody (10F10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26127

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26127-100ul
NBP3-26127-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10F10

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human CA125/MUC16 [UniProt Q8WXI7]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CA125/MUC16 Antibody (10F10) - BSA Free

CA125/MUC16 Antibody (10F10)

Immunohistochemistry: CA125/MUC16 Antibody (10F10) [NBP3-26127] -

Immunohistochemistry: CA125/MUC16 Antibody (10F10) [NBP3-26127] - Image of CA125/MUC16 Antibody (10F10) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for CA125/MUC16 Antibody (10F10) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CA125/MUC16

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Gene Symbol

MUC16

Additional CA125/MUC16 Products

Product Documents for CA125/MUC16 Antibody (10F10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CA125/MUC16 Antibody (10F10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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