CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72336

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CA125/MUC16 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 10329-10628 of human MUC16 (NP_078966) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free

Immunohistochemistry: CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free [NBP2-72336]

Immunohistochemistry: CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free [NBP2-72336]

Immunohistochemistry: CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free [NBP2-72336] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free [NBP2-72336]

Flow Cytometry: CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free [NBP2-72336]

Flow Cytometry: CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free [NBP2-72336] - E.coli lysate (left lane) and E.coli lysate expressing Human recombinant protein fragment (right lane) corresponding to amino acids 10329-10628 of human MUC16 (NP_078966) were separated by SDS-PAGE and immunoblotted with MUC16.

Applications for CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CA125/MUC16

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Gene Symbol

MUC16

Additional CA125/MUC16 Products

Product Documents for CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CA125/MUC16 Antibody (OTI2C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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