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CA125/MUC16 Antibody (X306cc)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-10032

Novus Biologicals, part of Bio-Techne
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NB120-10032
NB120-10032-0.2mg

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Microarray, Sandwich ELISA, Western Blot

Cited:

ELISA, MA, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # X306cc

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).

Epitope

A1 epitope group

Localization

Cell membrane; Single pass type I membrane protein. Secreted, extracellular space.

Specificity

CA125 antigen. Epitope specificity group A (ISOBM classification) similar to OC125.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CA125/MUC16 Antibody (X306cc)

Application
Recommended Usage

ELISA

1:100-1:2000

Sandwich ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Microarray was reported in scientific literature. Use in ELISA reported in scientific literature (PMID 24257318)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CA125/MUC16

CA125/MUC16 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that CA125/MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Entrez Gene IDs

94025 (Human)

Gene Symbol

MUC16

UniProt

Additional CA125/MUC16 Products

Product Documents for CA125/MUC16 Antibody (X306cc)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CA125/MUC16 Antibody (X306cc)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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