Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 16A

Format

BSA Free

Concentration

1 mg/ml

View all Cadherin-11 Antibodies »

Product Specifications

Immunogen

This antibody is a mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with affinity purified extracellular domain of Human cadherin-11-GST fusion protein.

Specificity

This antibody recognizes the extracellular domain of cadherin-11.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Cadherin-11 Antibody (16A) - BSA Free

Western Blot: Cadherin-11 Antibody (16A)BSA Free [NBP1-97676]

Western Blot: Cadherin-11 Antibody (16A)BSA Free [NBP1-97676]

Western Blot: Cadherin-11 Antibody (16A) [NBP1-97676] - Western blot on a lysate of Cadherin 11 transfected PC3 cells
Immunocytochemistry/ Immunofluorescence: Cadherin-11 Antibody (16A) - BSA Free [NBP1-97676]

Immunocytochemistry/ Immunofluorescence: Cadherin-11 Antibody (16A) - BSA Free [NBP1-97676]

Immunocytochemistry/Immunofluorescence: Cadherin-11 Antibody (16A) [NBP1-97676] - Immunocytochemistry on Cadherin 11 transfected PC3 cells

Applications for Cadherin-11 Antibody (16A) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:25-1:50

Western Blot

1:25-1:250
Application Notes
This antibody is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cadherin-11

Cadherin-11 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. Cadherin-11 is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Gene Symbol

CDH11

Additional Cadherin-11 Products

Product Documents for Cadherin-11 Antibody (16A) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cadherin-11 Antibody (16A) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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