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Cadherin-11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15661

Novus Biologicals, part of Bio-Techne
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NBP2-15661

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Chicken (96%), Porcine (99%), Rat (100%), Rhesus Macaque (99%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cadherin-11. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (92%), Zebrafish (82%).

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cadherin-11 Antibody

Immunohistochemistry-Frozen: Cadherin-11 Antibody [NBP2-15661]

Immunohistochemistry-Frozen: Cadherin-11 Antibody [NBP2-15661]

Immunohistochemistry-Frozen: Cadherin-11 Antibody [NBP2-15661] - Frozen-sectioned adult mouse cerebellum. Green: CDH11 stained by CDH11 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.
Cadherin-11 Antibody

Western Blot: Cadherin-11 Antibody [NBP2-15661] -

Western Blot: Cadherin-11 Antibody [NBP2-15661] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cadherin-11 antibody (NBP2-15661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Cadherin-11 Antibody

Western Blot: Cadherin-11 Antibody [NBP2-15661] -

Western Blot: Cadherin-11 Antibody [NBP2-15661] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Cadherin-11 antibody (NBP2-15661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Cadherin-11 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cadherin-11

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. [provided by RefSeq]

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Gene Symbol

CDH11

UniProt

Additional Cadherin-11 Products

Product Documents for Cadherin-11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cadherin-11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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