Cadherin-6/KCAD Antibody (5N2K3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16464

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16464-100ul
NBP3-16464-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5N2K3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cadherin-6/KCAD Antibodies »

Product Summary for Cadherin-6/KCAD Antibody (5N2K3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 691-790 of human Cadherin-6/KCAD (P55285). DIVPEALFLPRRTPTARDNTDVRDFINQRLKENDTDPTAPPYDSLATYAYEGTGSVADSLSSLESVTTDADQDYDYLSDWGPRFKKLADMYGGVDSDKDS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cadherin-6/KCAD Antibody (5N2K3)

Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464]

Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464]

Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Western blot analysis of extracts of various cell lines, using Cadherin-6/KCAD Rabbit mAb (NBP3-16464) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464]

Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464]

Western Blot: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Western blot analysis of extracts of various cell lines, using Cadherin-6/KCAD Rabbit mAb (NBP3-16464) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464]

Immunoprecipitation: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464]

Immunoprecipitation: Cadherin-6/KCAD Antibody (5N2K3) [NBP3-16464] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug Cadherin-6/KCAD antibody (NBP3-16464). Western blot was performed from the immunoprecipitate using Cadherin-6/KCAD antibody (NBP3-16464) at a dilition of 1:1000.

Applications for Cadherin-6/KCAD Antibody (5N2K3)

Application
Recommended Usage

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cadherin-6/KCAD

K Cadherin encodes a type II classical cadherin from the cadherin superfamily. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types and the maintenance of orderly structures such as epithelium. Strong transcriptional expression of this gene has been observed in hepatocellular and renal carcinoma cell lines, suggesting a possible role in metastasis and invasion.

Alternate Names

Cadherin6, CDH6, K-Cadherin

Gene Symbol

CDH6

Additional Cadherin-6/KCAD Products

Product Documents for Cadherin-6/KCAD Antibody (5N2K3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cadherin-6/KCAD Antibody (5N2K3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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