Calbindin D-28K Antibody (1K2K2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16209

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16209-100ul
NBP3-16209-20ul
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1K2K2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calbindin D-28K Antibodies »

Product Summary for Calbindin D-28K Antibody (1K2K2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Calbindin D-28K (P05937). MAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQLKSC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calbindin D-28K Antibody (1K2K2)

Western Blot: Calbindin D-28K Antibody (1K2K2) [NBP3-16209]

Western Blot: Calbindin D-28K Antibody (1K2K2) [NBP3-16209]

Western Blot: Calbindin D-28K Antibody (1K2K2) [NBP3-16209] - Western blot analysis of extracts of various cell lines, using Calbindin D-28K Rabbit mAb (NBP3-16209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Calbindin D-28K Antibody (1K2K2)

Immunohistochemistry: Calbindin D-28K Antibody (1K2K2) [NBP3-16209] -

Immunohistochemistry: Calbindin D-28K Antibody (1K2K2) [NBP3-16209] - Confocal imaging of human kidney using Calbindin Rabbit mAb (dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Applications for Calbindin D-28K Antibody (1K2K2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calbindin D-28K

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Alternate Names

CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type

Gene Symbol

CALB1

Additional Calbindin D-28K Products

Product Documents for Calbindin D-28K Antibody (1K2K2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calbindin D-28K Antibody (1K2K2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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