Calbindin D-28K Antibody (4H7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50048

Novus Biologicals, part of Bio-Techne
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NBP2-50048
NBP2-50048SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4H7

Concentration

1 mg/ml

View all Calbindin D-28K Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Calbindin D-28K. [UniProt# P05937]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calbindin D-28K Antibody (4H7)

Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048]

Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048]

Western Blot: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of cow cerebellum lysate were probed with NBP2-50048. NBP2-50048 binds strongly and specifically to Calbindin at 28 kDa.
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048]

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048]

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Rat brain cerebellum (Middle) and cortex (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were co-stained with NBP2-50048 (red) and anti-MeCP2 rabbit antibody (NBP2-50060, green). Calbindin is predominantly expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum, and selectively expressed in certain type of interneurons (calbindin-postive interneuron) in the cortex. MeCP2 is universally expressed in the nuclei of almost all neurons. As a result, calbindin-expressing cell is strongly labeled with red in soma, but the nucleus appears to be yellow. Blue is Dapi nucleus staining. Insets are high magnification images of the boxed areas.
Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (4H7) [NBP2-50048]

Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (4H7) [NBP2-50048]

Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (4H7) [NBP2-50048] - Analysis of rat brain cerebellum section stained with mouse mAb to calbindin, NBP2-50048, dilution 1:1,000, in green, and costained with rabbit pAb to calretinin, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. Calbindin antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum. In contrast the calretinin antibody stains granule cells, in the granualar layer, and their processes in the molecular layer.

Applications for Calbindin D-28K Antibody (4H7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Immunohistochemistry Free-Floating

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000-1:5000
Application Notes
Use in IHC-P reported in scientific literature (PMID: 29208685).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calbindin D-28K

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Alternate Names

CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type

Gene Symbol

CALB1

UniProt

P05937

Additional Calbindin D-28K Products

Product Documents for Calbindin D-28K Antibody (4H7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calbindin D-28K Antibody (4H7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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