Calbindin D-28K Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86664

Novus Biologicals, part of Bio-Techne
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NBP1-86664
NBP1-86664-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calbindin D-28K Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRCQQ

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calbindin D-28K Antibody

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664]

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664]

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining in human kidney and lymph node tissues using anti-CALB1 antibody. Corresponding CALB1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664]

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664]

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP1-86664] - Staining of human cerebellum, kidney, liver and lymph node using Anti-CALB1 antibody NBP1-86664 (A) shows similar protein distribution across tissues to independent antibody NBP2-38798 (B).
Western Blot: Calbindin D-28K Antibody [NBP1-86664]

Western Blot: Calbindin D-28K Antibody [NBP1-86664]

Western Blot: Calbindin D-28K Antibody [NBP1-86664] - Analysis in human kidney tissue.
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Applications for Calbindin D-28K Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calbindin D-28K

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Alternate Names

CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type

Gene Symbol

CALB1

Additional Calbindin D-28K Products

Product Documents for Calbindin D-28K Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calbindin D-28K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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