Calcineurin B Antibody (4E1)
Novus Biologicals, part of Bio-Techne | Catalog # H00005534-M01
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4E1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PPP3R1 (AAH27913, 1 a.a. ~ 170 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV
Specificity
PPP3R1 - protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Calcineurin B Antibody (4E1)
![Western Blot: Calcineurin B Antibody (4E1) [H00005534-M01]](https://resources.bio-techne.com/images/products/Calcineurin-B-Antibody-4E1-Western-Blot-H00005534-M01-img0006.jpg "Western Blot: Calcineurin B Antibody (4E1) [H00005534-M01]")
Western Blot: Calcineurin B Antibody (4E1) [H00005534-M01]
Western Blot: Calcineurin B Antibody (4E1) [H00005534-M01] - PPP3R1 monoclonal antibody (M01), clone 4E1. Analysis of PPP3R1 expression in rat brain.![Immunocytochemistry/ Immunofluorescence: Calcineurin B Antibody (4E1) [H00005534-M01]](https://resources.bio-techne.com/images/products/Calcineurin-B-Antibody-4E1-Immunocytochemistry-Immunofluorescence-H00005534-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Calcineurin B Antibody (4E1) [H00005534-M01]")
Immunocytochemistry/ Immunofluorescence: Calcineurin B Antibody (4E1) [H00005534-M01]
Immunocytochemistry/Immunofluorescence: Calcineurin B Antibody (4E1) [H00005534-M01] - Analysis of monoclonal antibody to PPP3R1 on HeLa cell. Antibody concentration 25 ug/ml.![ELISA: Calcineurin B Antibody (4E1) [H00005534-M01]](https://resources.bio-techne.com/images/products/Calcineurin-B-Antibody-4E1-ELISA-H00005534-M01-img0007.jpg "ELISA: Calcineurin B Antibody (4E1) [H00005534-M01]")
ELISA: Calcineurin B Antibody (4E1) [H00005534-M01]
ELISA: Calcineurin B Antibody (4E1) [H00005534-M01] - Calcineurin B Antibody (4E1) [H00005534-M01] - Detection limit for recombinant GST tagged PPP3R1 is approximately 0.1ng/ml as a capture antibody.Applications for Calcineurin B Antibody (4E1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IF and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calcineurin B
Alternate Names
CALNB1, Cnb1, MCIP1, PP2B beta 1, PPP3R1
Entrez Gene IDs
5534 (Human)
Gene Symbol
PPP3R1
OMIM
601302 (Human)
UniProt
Additional Calcineurin B Products
Product Documents for Calcineurin B Antibody (4E1)
Product Specific Notices for Calcineurin B Antibody (4E1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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