Calcium-binding-protein-P22 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33736
![Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736]](https://resources.bio-techne.com/images/products/Calcium-binding-protein-P22-Antibody-Western-Blot-NBP1-33736-img0004.jpg "Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Predicted:
Bovine (99%), Chicken (96%), Rhesus Macaque (100%), Xenopus (90%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calcium-binding-protein-P22. The exact sequence is proprietary.
Reactivity Notes
Zebrafish (86%), Xenopus laevis (90%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calcium-binding-protein-P22 Antibody
![Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736]](https://resources.bio-techne.com/images/products/Calcium-binding-protein-P22-Antibody-Western-Blot-NBP1-33736-img0006.jpg "Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736]")
Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736]
Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGE![Immunocytochemistry/ Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736]](https://resources.bio-techne.com/images/products/Calcium-binding-protein-P22-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33736-img0009.jpg "Immunocytochemistry/ Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736]")
Immunocytochemistry/ Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736]
Immunocytochemistry/Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Mouse lung. Calcium binding protein P22 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Mouse lung. Calcium binding protein P22 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.![Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736]](https://resources.bio-techne.com/images/products/Calcium-binding-protein-P22-Antibody-Western-Blot-NBP1-33736-img0005.jpg "Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736]")
Applications for Calcium-binding-protein-P22 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Application Notes
IP-Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calcium-binding-protein-P22
Alternate Names
Calcineurin B homolog, Calcineurin homologous protein, calcium binding protein P22, Calcium-binding protein CHP, calcium-binding protein p22, SLC9A1 binding protein, SLC9A1BP
Gene Symbol
CHP1
Additional Calcium-binding-protein-P22 Products
Product Documents for Calcium-binding-protein-P22 Antibody
Product Specific Notices for Calcium-binding-protein-P22 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.