Calcium-binding-protein-P22 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35602
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human Calcium-binding-protein-P22 (NP_009167.1).
Sequence:
MGSRASTLLRDEELEEIKKETGFSHSQITRLYSRFTSLDKGENGTLSREDFQRIPELAINPLGDRIINAFFPEGEDQVNFRGFMRTLAHFRPIEDNEKSKDVNGPEPLNSRSNKLHFAFRLYDLDKDEKISRDELLQVLRMMVGVNISDEQLGSIADRTIQEADQDGDSAISFTEFVKVLEKVDVEQKMSIRFLH
Sequence:
MGSRASTLLRDEELEEIKKETGFSHSQITRLYSRFTSLDKGENGTLSREDFQRIPELAINPLGDRIINAFFPEGEDQVNFRGFMRTLAHFRPIEDNEKSKDVNGPEPLNSRSNKLHFAFRLYDLDKDEKISRDELLQVLRMMVGVNISDEQLGSIADRTIQEADQDGDSAISFTEFVKVLEKVDVEQKMSIRFLH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calcium-binding-protein-P22 Antibody
Immunocytochemistry/ Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP3-35602] -
Immunocytochemistry/ Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP3-35602] - Immunofluorescence analysis of L929 cells using Calcium-binding-protein-P22 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Western Blot: Calcium-binding-protein-P22 Antibody [NBP3-35602] -
Western Blot: Calcium-binding-protein-P22 Antibody [NBP3-35602] - Western blot analysis of various lysates using Calcium-binding-protein-P22 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP3-35602] -
Immunocytochemistry/ Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP3-35602] - Immunofluorescence analysis of U-2 OS cells using Calcium-binding-protein-P22 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for Calcium-binding-protein-P22 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Calcium-binding-protein-P22
Alternate Names
Calcineurin B homolog, Calcineurin homologous protein, calcium binding protein P22, Calcium-binding protein CHP, calcium-binding protein p22, SLC9A1 binding protein, SLC9A1BP
Gene Symbol
CHP1
Additional Calcium-binding-protein-P22 Products
Product Documents for Calcium-binding-protein-P22 Antibody
Product Specific Notices for Calcium-binding-protein-P22 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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