Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-19347

Novus Biologicals, part of Bio-Techne
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NB120-19347
NB120-19347SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5C10, ADD

Format

BSA Free

Concentration

1 mg/ml

View all Calcium-sensing R/CaSR Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide corresponding to residues 214 - 235 of human calcium sensing receptor R / CaSR [UniProt P41180]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21969374). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Porcine reactivity reported in scientific literature (PMID: 27836810).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD)BSA Free [NB120-19347]

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD)BSA Free [NB120-19347]

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Total protein from human Hek293 and rat Pancreas was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CaSR in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of primary astrocytes using Calcium-sensing R/CaSR antibody. Primary astrocytes co-stained with cilia (Arl13b, red, top left), CASR (green, bottom left), DAPI (blue, top right) and merged (bottom right). Image from verified customer review.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human kidney tissue.
View 4 more images

Applications for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500. Use reported in scientific literature

Western Blot

2 ug/ml
Application Notes
In Western blot this antibody detects bands at approximately 130 and 150 kDa. We have conflicting data on the use of this antibody in Flow.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB120-19347 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calcium-sensing R/CaSR

The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).

Long Name

Calcium-sensing Receptor

Alternate Names

CAR, CaSR, EIG8, FHH, FIH, GPRC2A, HHC1, NSHPT, PCaR1

Entrez Gene IDs

846 (Human); 24247 (Rat)

Gene Symbol

CASR

UniProt

P41180 (Rat)

Additional Calcium-sensing R/CaSR Products

Product Documents for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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