Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47816

Novus Biologicals, part of Bio-Techne
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NBP2-47816-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # h-CALD

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Caldesmon/CALD1 Antibodies »

Product Summary for Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free

Immunogen

Crude human uterus extract

Localization

Cytoplasmic

Specificity

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (12080kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This monoclonal antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Marker

Smooth Muscle Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44421).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free

Western Blot: Caldesmon/CALD1 Antibody (h-CALD)Azide and BSA Free [NBP2-47816]

Western Blot: Caldesmon/CALD1 Antibody (h-CALD)Azide and BSA Free [NBP2-47816]

Western Blot: Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free [NBP2-47816] - Western Blot Analysis of human Ovary tissue using Caldesmon/CALD1 antibody (h-CALD).
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free [NBP2-47816]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free [NBP2-47816]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free [NBP2-47816] - Human Uterus stained with Caldesmon Monoclonal Antibody (h-CALD).
Simple Western: Caldesmon/CALD1 Antibody (h-CALD)Azide and BSA Free [NBP2-47816]

Simple Western: Caldesmon/CALD1 Antibody (h-CALD)Azide and BSA Free [NBP2-47816]

Simple Western: Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free [NBP2-47816] - Electropherogram image of the corresponding Simple Western lane. Caldesmon/CALD1 antibody was used at 10 ug/ml dilution of h. Aorta and h. Uterus lysates(s) respectively.
View 1 more images

Applications for Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

0.25 - 0.5 ug/ml

Simple Western

10 ug/ml

Western Blot

2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Caldesmon/CALD1

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Gene Symbol

CALD1

Additional Caldesmon/CALD1 Products

Product Documents for Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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