Caldesmon/CALD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85701

Novus Biologicals, part of Bio-Techne
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NBP1-85701
NBP1-85701-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Caldesmon/CALD1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MTHKLKHTENTFSRPGGRASVDTKEAEGAPQVEAGKRLEELRRRRGETESEEFEKLKQKQQEAALELEELKKKREERRKVLEEEEQRRKQEEADRKLREEEEKRRLKEEIERRRAEAAEKRQKMPEDGLSDD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caldesmon/CALD1 Antibody

Analysis in human prostate and skeletal muscle tissues using NBP1-85701 antibody. Corresponding CALD1 RNA-seq data are presented for the same tissues.

Analysis in human prostate and skeletal muscle tissues using NBP1-85701 antibody. Corresponding CALD1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody [NBP1-85701] - Staining of human cerebral cortex, placenta, skeletal muscle and smooth muscle using Anti-CALD1 antibody NBP1-85701 (A) shows similar protein distribution across tissues to independent antibody NBP1-85702 (B).
Western Blot: Caldesmon/CALD1 Antibody [NBP1-85701]

Western Blot: Caldesmon/CALD1 Antibody [NBP1-85701]

Western Blot: Caldesmon/CALD1 Antibody [NBP1-85701] - Western blot analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
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Applications for Caldesmon/CALD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caldesmon/CALD1

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Gene Symbol

CALD1

Additional Caldesmon/CALD1 Products

Product Documents for Caldesmon/CALD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caldesmon/CALD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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