Calmodulin 2 Antibody (J4D8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21504
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # J4D8
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Calmodulin (1-149aa) purified from E. coli (Accesion #: NP_001734; Uniprot #: P0DP24).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Calmodulin 2 Antibody (J4D8) - BSA Free
Western Blot: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] -
Western Blot: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-21504 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: NIH3T3 cell lysate. Lane 2: HeLa cell lysate. Lane 3: Jurkat cell lysate. Lane 4: A549 cell lysate. Lane 5: MCF7 cell lysate. Lane 6: Ramos cell lysate. Lane 7: PC3 cell lysate
Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] -
Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - ICC/IF analysis of Calmodulin 2 in HeLa cells. The cell was stained with NBP3-21504 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] -
Flow Cytometry: Calmodulin 2 Antibody (J4D8) - BSA Free [NBP3-21504] - Flow cytometry analysis of Calmodulin 2 in HeLa cells. The cell was stained with NBP3-21504 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Applications for Calmodulin 2 Antibody (J4D8) - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calmodulin 2
Alternate Names
CALM, CALM1, CALM3, CALML2, calmodulin, calmodulin 2 (phosphorylase kinase, delta), CAM, CAM1, CAM2, CAM3, CAMB, CAMC, CAMII, CAMIII, FLJ99410, LP7057 protein, PHKD, PHKD2, phosphorylase kinase delta
Gene Symbol
CALM2
Additional Calmodulin 2 Products
Product Documents for Calmodulin 2 Antibody (J4D8) - BSA Free
Product Specific Notices for Calmodulin 2 Antibody (J4D8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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