Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AF18

Format

BSA Free

Concentration

1 mg/ml

View all Calnexin Antibodies »

Product Specifications

Immunogen

Focus human hepatoma cell lysate.

Localization

Endoplasmic reticulum

Specificity

Calnexin (AF18)

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Calnexin Antibody (AF18) - BSA Free

Western Blot: Calnexin Antibody (AF18)BSA Free [NB300-518]

Western Blot: Calnexin Antibody (AF18)BSA Free [NB300-518]

Western Blot: Calnexin Antibody (AF18) [NB300-518] - Analysis of 25 ug of HepG2 lysate.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (AF18) - BSA Free [NB300-518]

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (AF18) - BSA Free [NB300-518]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (AF18) [NB300-518] - Staining of calnexin (red) in U87 cells.
Immunohistochemistry-Paraffin: Calnexin Antibody (AF18) - BSA Free [NB300-518]

Immunohistochemistry-Paraffin: Calnexin Antibody (AF18) - BSA Free [NB300-518]

Immunohistochemistry-Paraffin: Calnexin Antibody (AF18) [NB300-518] - Both normal and cancer biopsies of deparaffinized Human hepatocarcinoma tissues.
View 4 more images

Applications for Calnexin Antibody (AF18) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:20

Immunohistochemistry-Paraffin

1:20

Immunoprecipitation

5 ug

Western Blot

1:100-1:1000
Application Notes
Detects an approx. 90 kDa band representing calnexin in human liver, skin, adrenal gland, kidney, heart, colon, testes and ovary extracts. ICC: Staining of calnexin in Huh-7 cells yields a pattern consistent with specific endoplasmic reticulum staining.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 and protein disulfide isomerase. (See catalog #

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90

Entrez Gene IDs

821 (Human)

Gene Symbol

CANX

Additional Calnexin Products

Product Documents for Calnexin Antibody (AF18) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Calnexin Antibody (AF18) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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